کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6818020 | 546997 | 2016 | 27 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
A comparison of salivary testosterone measurement using immunoassays and tandem mass spectrometry
ترجمه فارسی عنوان
مقایسه اندازه گیری تستوسترون بزاق با استفاده از ایمونواسیون و اسپکترومتراسیون توده دو طرفه
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کلمات کلیدی
تستوسترون بزاق، آزمایشات ایمنی، کروماتوگرافی مایع طیف سنجی جرمی، اندازه گیری،
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
علوم غدد
چکیده انگلیسی
Enzyme immunoassays (EIAs) are widely used to measure salivary testosterone. However, little is known about how accurately different EIAs assess testosterone, partially because estimates across various EIAs differ considerably. We compared testosterone concentrations across EIAs of three commonly used manufacturers (DRG International, Salimetrics, and IBL International) to liquid chromatography tandem mass spectrometry (LC-MS/MS). Relative to EIAs from Salimetrics and IBL International, EIAs supplied by DRG International provided the closest approximation to LC-MS/MS testosterone concentrations, followed closely by EIAs from Salimetrics, and then IBL. Additionally, EIAs tended to inflate estimates of lower testosterone concentrations in women. Examining our results and comparing them to existing data revealed that testosterone EIAs had decreased linear correspondence with LC-MS/MS in comparison to cortisol EIAs. Overall, this paper provides researchers with information to better measure testosterone in their research and more accurately compare testosterone measurements across different methods.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Psychoneuroendocrinology - Volume 71, September 2016, Pages 180-188
Journal: Psychoneuroendocrinology - Volume 71, September 2016, Pages 180-188
نویسندگان
Keith M. Welker, Bethany Lassetter, Cassandra M. Brandes, Smrithi Prasad, Dennis R. Koop, Pranjal H. Mehta,