کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
682197 888976 2010 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Identification of two flavin monooxygenases from an effluent treatment plant sludge metagenomic library
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی تکنولوژی و شیمی فرآیندی
پیش نمایش صفحه اول مقاله
Identification of two flavin monooxygenases from an effluent treatment plant sludge metagenomic library
چکیده انگلیسی

Oxygenases are useful for the production of many industrially important molecules. Screening of an effluent treatment plant (ETP) sludge metagenomic library identified two clones encoding proteins, B1 and B2, with similarity to putative flavin monooxygenases from Mesorhizobium loti and Sphingomonas wittichi, respectively. The deduced amino acid sequences show only 20% identity, but both have a paired Rossman fold and a flavin monooxygenase (FMO) motif. B1 and B2 appear to be members of the flavin-containing monooxygenase and the Baeyer–Villiger monooxygenases subfamilies, respectively. When expressed in Escherichia coli, the two clones produced activities that oxidized indole to a mixture of indigo and indirubin pigments. These results suggest that B1 and B2 have potential as a biocatalyst in indigo/indirubin production.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Bioresource Technology - Volume 101, Issue 21, November 2010, Pages 8481–8484
نویسندگان
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