کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
682286 | 888982 | 2011 | 7 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Characterization of a copper-resistant symbiotic bacterium isolated from Medicago lupulina growing in mine tailings Characterization of a copper-resistant symbiotic bacterium isolated from Medicago lupulina growing in mine tailings](/preview/png/682286.png)
A root nodule bacterium, Sinorhizobium meliloti CCNWSX0020, resistant to 1.4 mM Cu2+ was isolated from Medicago lupulina growing in mine tailings. In medium supplied with copper, this bacterium showed cell deformation and aggregation due to precipitation of copper on the cell surface. Genes similar to the copper-resistant genes, pcoR and pcoA from Escherichia coli, were amplified by PCR from a 1.4-Mb megaplasmid. Inoculation with S. meliloti CCNWSX0020 increased the biomass of M. lupulina grown in medium added 0 and 100 mg Cu2+ kg−1 by 45.8% and 78.2%, respectively, and increased the copper concentration inside the plant tissues grown in medium supplied with 100 μM Cu2+ by 39.3%, demonstrating that it is a prospective symbiotic system for bioremediation purposes.
Journal: Bioresource Technology - Volume 102, Issue 2, January 2011, Pages 703–709