کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
682705 | 888989 | 2010 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Production of cytidine 5′-diphosphorylcholine with high utilization of ATP by whole cells of Saccharomycescerevisiae
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
تکنولوژی و شیمی فرآیندی
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چکیده انگلیسی
Cytidine 5′-diphosphorylcholine (CDP-choline) was produced using a high efficiency ATP regeneration system and the Kennedy pathway in whole cells of Saccharomycescerevisiae As 2.398. Out of eight variables, KH2PO4, glycerol and (NH4)2SO4 were considered to be the most significant factors by response surface methodology including a Plackett–Burman design, path of steepest accent and central composite design. The optimum levels of the three variables were 20.13 g/L KH2PO4, 12.35 g/L glycerol and 0.49 g/L (NH4)2SO4, respectively. Energy utilization efficiency increased from 10.59% to 16.72% and choline chloride conversion yields increased from 12.35% to 42.78%. A high efficiency ATP regeneration system improves CDP-choline production.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Bioresource Technology - Volume 101, Issue 22, November 2010, Pages 8807–8813
Journal: Bioresource Technology - Volume 101, Issue 22, November 2010, Pages 8807–8813
نویسندگان
Jiapeng Tang, Yong Chen, Xiaochun Chen, Yuelan Yao, Hanjie Ying, Jian Xiong, Jianxin Bai,