کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
683050 | 888994 | 2010 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Enzymatic synthesis of eugenol benzoate by immobilized Staphylococcus aureus lipase: Optimization using response surface methodology and determination of antioxidant activity
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
تکنولوژی و شیمی فرآیندی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Enzymatic synthesis of eugenol benzoate by immobilized Staphylococcus aureus lipase: Optimization using response surface methodology and determination of antioxidant activity Enzymatic synthesis of eugenol benzoate by immobilized Staphylococcus aureus lipase: Optimization using response surface methodology and determination of antioxidant activity](/preview/png/683050.png)
چکیده انگلیسی
The ability of a non-commercial immobilized Staphylococcus aureus lipase to catalyze the esterification of eugenol with benzoic acid was checked and the antioxidant power of the ester formed was evaluated. Response surface methodology based on four variables (the reaction temperature, the amount of lipase, the benzoic acid/eugenol molar ratio and the volume of solvent) was used to optimize the experimental conditions of eugenol benzoate synthesis. The maximum conversion yield (75%) was obtained using 240 IU of immobilized lipase, a benzoic acid/eugenol molar ratio of 1.22 dissolved in 4.6 ml chloroform at 41 °C. The antioxidant activities of eugenol and its ester were evaluated. Compared to BHT, used as a model synthetic antioxidant, the eugenol benzoate showed a higher antioxidative activity. The IC50 value for 1,1-diphenyl-2-picrylhydrazyl was found to be 18.2 μg/ml versus 20.2 μg/ml for eugenol and eugenol benzoate.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Bioresource Technology - Volume 101, Issue 8, April 2010, Pages 2809-2817
Journal: Bioresource Technology - Volume 101, Issue 8, April 2010, Pages 2809-2817
نویسندگان
Habib Horchani, Nadia Ben Salem, Zied Zarai, Adel Sayari, Youssef Gargouri, Moncef Chaâbouni,