کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
683591 889003 2009 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
In situ preparation of magnetic Fe3O4-chitosan nanoparticles for lipase immobilization by cross-linking and oxidation in aqueous solution
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی تکنولوژی و شیمی فرآیندی
پیش نمایش صفحه اول مقاله
In situ preparation of magnetic Fe3O4-chitosan nanoparticles for lipase immobilization by cross-linking and oxidation in aqueous solution
چکیده انگلیسی

A new and simple method has been proposed to prepare magnetic Fe3O4-chitosan (CS) nanoparticles by cross-linking with sodium tripolyphosphate (TPP), precipitation with NaOH and oxidation with O2 in hydrochloric acid aqueous phase containing CS and Fe(OH)2, and these magnetic CS nanoparticles were used to immobilize lipase. The effects on the sequence of adding NaOH and TPP, the reaction temperature, and the ratio of CS/Fe(OH)2 were studied. TEM showed that the diameter of composite nanoparticles was about 80 nm, and that the magnetic Fe3O4 nanoparticles with a diameter of 20 nm were evenly dispersed in the CS materials. Magnetic measurement revealed that the saturated magnetisation of the Fe3O4-CS nanoparticles could reach 35.54 emu/g. The adsorption capacity of lipase onto nanoparticles could reach 129 mg/g; and the maximal enzyme activity was 20.02 μmol min−1 mg−1 (protein), and activity retention was as high as 55.6% at a certain loading amount.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Bioresource Technology - Volume 100, Issue 14, July 2009, Pages 3459–3464
نویسندگان
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