کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
683779 | 889006 | 2009 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Medium optimization for the production of cyclic adenosine 3â²,5â²-monophosphate by Microbacterium sp. no. 205 using response surface methodology
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
تکنولوژی و شیمی فرآیندی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Response surface methodology was employed to optimize medium composition for the production of cyclic adenosine 3â²,5â²-monophosphate (cAMP) with Microbacterium sp. no. 205. A fractional factorial design (211 â 7) was applied to evaluate the effects of different components in the medium. K2HPO4, MgSO4 and NaF were found to significantly influence on the cAMP production. The steepest ascent method was used to access the optimal region of the medium composition. The concentrations of the three factors were optimized subsequently using central composite design and response surface methodology. The optimal medium composition to achieve the optimal cAMP production was determined (g/L): K2HPO4, 12.78; MgSO4, 3.53 and NaF, 0.18. The corresponding cAMP concentration was 8.50 g/L, which was about 1.8-fold increase compared with that using the original medium. Validation experiments were also carried out to prove the adequacy and the accuracy of the model obtained. The cAMP fermentation in 5 L fermenter reached 9.87 g/L.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Bioresource Technology - Volume 100, Issue 2, January 2009, Pages 919-924
Journal: Bioresource Technology - Volume 100, Issue 2, January 2009, Pages 919-924
نویسندگان
Xiao-Chun Chen, Jian-Xin Bai, Jia-Ming Cao, Zhen-Jiang Li, Jian Xiong, Lei Zhang, Yuan Hong, Han-Jie Ying,