کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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683894 | 889008 | 2010 | 8 صفحه PDF | دانلود رایگان |
Supercritical fluid carbon dioxide (SF-CO2) extraction (SFE) of seed oil from yellow horn and its anti-oxidant activity were investigated. The effects of CO2 flow rate and particle size were firstly optimized, and a central composite design (CCD) combined with response surface methodology was used to study the effects of extraction pressure, temperature and time on the extraction yields. A maximal extraction yield of 61.28% was achieved under optimal conditions of extraction pressure 30 MPa at 45.68 °C, 2.08 h and CO2 flow rate 12 kg/h with 0.5 mm particle size. By analyzing the chemical composition of the seed oil, we found that the content of unsaturated fatty acids was approximately 90%. Furthermore, the anti-oxidant activity of seed oil was assessed by a 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical-scavenging assay and a β-carotene bleaching test. Yellow horn seed oil possessed notable concentration-dependent anti-oxidant activity with IC50 values of 0.151 and 0.195 g/mL, respectively.
Journal: Bioresource Technology - Volume 101, Issue 7, April 2010, Pages 2537–2544