کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
685028 889032 2008 12 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Xylanase production by Burkholderia sp. DMAX strain under solid state fermentation using distillery spent wash
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی تکنولوژی و شیمی فرآیندی
پیش نمایش صفحه اول مقاله
Xylanase production by Burkholderia sp. DMAX strain under solid state fermentation using distillery spent wash
چکیده انگلیسی

Xylanase production by a newly isolated strain of Burkholderia sp. was studied under solid state fermentation using anaerobically treated distillery spent wash. Response surface methodology (RSM) involving Box–Behnken design was employed for optimizing xylanase production. The interactions between distillery effluent concentration, initial pH, moisture ratio and inoculum size were investigated and modeled. Under optimized conditions, xylanase production was found to be in the range of 5200–5600 U/g. The partially purified enzyme recovered after ammonium sulphate fractionation showed maximum activity at 50 °C and pH 8.6. Kinetic parameters like Km and Vmax for xylan were found to be 12.75 mg/ml and 165 μmol/mg/min. In the presence of metal ions such as Ca2+, Co2+, Mn2+, Ba2+, Mg2+ and protein disulphide reducing agents such as β-mercaptoethanol and dithiotheritol (DTT) the activity of enzyme increased, where as strong inhibition of enzyme activity was observed in the presence of Cu2+, Ag+, Fe2+ and SDS. The crude enzyme hydrolysed lignocellulosic substrate, wheat bran as well as industrial pulp.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Bioresource Technology - Volume 99, Issue 16, November 2008, Pages 7553–7564
نویسندگان
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