کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
685293 889037 2008 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Assessment of microbial diversity in effluent treatment plants by culture dependent and culture independent approaches
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی تکنولوژی و شیمی فرآیندی
پیش نمایش صفحه اول مقاله
Assessment of microbial diversity in effluent treatment plants by culture dependent and culture independent approaches
چکیده انگلیسی

Microbial community structure of two distinct effluent treatment plants (ETPs) of pesticide and pharmaceutical industries was assessed and defined by (i) culture dependent and culture independent approaches on the basis of 16S rRNA gene sequencing, (ii) diversity index analysis – operational taxonomic units (OTUs). A total of 38 and 44 bacterial OTUs having 85–99% similarity with the closest match in the database were detected among pharmaceutical and pesticide sludge samples, respectively. Fifty percent of the OTUs were related to uncultured bacteria. These OTUs had a Shannon diversity index value of 2.09–2.33 for culturables and in the range of 3.25–3.38 for unculturables. The high species evenness values of 0.86 and 0.95 indicated the vastness of microbial diversity retrieved by these approaches. The dominant cultured bacteria indicative of microbial diversity in functional ETPs were Alcaligenes, Bacillus and Pseudomonas. Brevundimonas, Citrobacter, Pandoraea and Stenotrophomonas were specific to pesticide ETP and Agrobacterium, Brevibacterium, Micrococcus, Microbacterium, Paracoccus and Rhodococcus were specific to pharmaceutical ETP. These microbes can thus be maintained and exploited for efficient functioning and maintenance of ETPs.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Bioresource Technology - Volume 99, Issue 15, October 2008, Pages 7098–7107
نویسندگان
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