Productivity of laccase in solid substrate fermentation of selected agro-residues by Pycnoporus sanguineus

The extraction of laccase was also optimized in this study. Recovery of laccase was fourfold higher at 30.6 U/g substrate on day 10 of SSF using unadjusted tap water at pH 8.0 as extraction medium at 25 ± 2 °C compared to laccase recovery of 7.46 U/g substrate using sodium acetate buffer at pH 4.8 at 4 °C. Further optimization showed that laccase recovery was increased by 50% with a value of 46.5 U/g substrate on day 10 of SSF when the extraction medium was tap water adjusted to pH 5.0 at 25 ± 2 °C.