کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
691466 | 1460430 | 2014 | 6 صفحه PDF | دانلود رایگان |
• The physico-chemical characterization of EPS fractions was determined by GC, FT-IR and TGA, etc.
• The EPS from STR was mainly composed of mannose and glucose, while purified EPS from AR mainly composed of glucose.
• The degradation temperature (Td) of the EPS from STR (152 °C) was higher than that of EPS from AR (116 °C).
• The EPS from STR exhibited the higher antioxidant ability than EPS from AR, which might be attributed to the molecular weight, monosaccharide composition and the configuration of glycosidic bond in the EPS groups.
In the present study, exopolysaccharides (EPS) production and pellet morphology of Pycnoporus sanguineus was investigated in a 5-L stirred-tank (STR) and a 5-L airlift reactors (AR). Maximum EPS production (2.92 g/L) was obtained after 4 days of cultivation in STR, whereas, EPS concentration was maximal (2.14 g/L) after 2 days in AR. The morphological form of the fungal pellets in AR was more hairy than those in the STR. Furthermore, monosaccharide composition analysis indicated that purified EPS from STR was mainly composed of mannose and glucose, while purified EPS from AR mainly composed of glucose. Then, FT-IR spectral analysis of the purified EPS revealed prominent characteristic groups. Dye-inclusion studies with Congo red indicated that each EPS existed in a random coil conformational state. Furthermore, thermo gravimetric analysis (TGA) indicated the degradation temperature (Td) of the EPS from STR (152 °C) was higher than that of EPS from AR (116 °C). In addition, the antioxidant activities of the polysaccharides from different bioreactors were investigated. EPS from STR exhibited the higher antioxidant ability than EPS from AR, which might be attributed to the molecular weight, monosaccharide composition and the configuration of glycosidic bond in the EPS groups.
Journal: Journal of the Taiwan Institute of Chemical Engineers - Volume 45, Issue 5, September 2014, Pages 2075–2080