کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
692094 1460509 2016 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Influences on the film thickness in the enzymatic autodeposition process of casein
ترجمه فارسی عنوان
تأثیر ضخامت فیلم در فرایند جذب آنزیمی کازئین
کلمات کلیدی
کازئین، پوشش بر پایه بنیادی، تنظیم خودکار، آنزیم، ضخامت فیلم، پروتئین
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی تکنولوژی و شیمی فرآیندی
چکیده انگلیسی


• A novel technique for the formation of biological coatings.
• An environmental friendly and controlled deposition of thin protein coatings.
• Coating of complex objects with biocompatible films.
• A model system for enzymatically initialized deposition of coatings.

A novel technique for the formation of biological coatings is presented. An enzymatic reaction is used to trigger the coagulation and specific deposition of protein particles on a support surface. As a model system, the major milk protein casein and the protease chymosin are used for a controlled deposition of casein biocoatings. The presented system is based on the natural enzymatic reaction used in cheesemaking and allows for a high control over the deposition process. For this purpose, chymosin is physically immobilized onto the support material and cleaves casein micelles in close proximity to the surface, initializing deposition of casein. This method shows characteristics of the autodeposition process of latex particles. Casein deposition takes place until the enzyme molecules are covered by cleaved casein micelles and are no longer able to cleave additional casein micelles. This paper reports the influence of reaction parameters such as reaction time, pH value, and casein concentration on the film thickness in the enzymatic autodeposition process. Film thickness of the deposited casein films increases up to 90 min of deposition time. After that, no significant increase of film thickness is observed. Higher casein concentrations and deposition in the acidic range of pH also result in higher film thickness.

Figure optionsDownload as PowerPoint slide

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Progress in Organic Coatings - Volume 94, May 2016, Pages 56–61
نویسندگان
, , , ,