کد مقاله کد نشریه سال انتشار مقاله انگلیسی ترجمه فارسی نسخه تمام متن
69296 48738 2016 8 صفحه PDF سفارش دهید دانلود کنید
عنوان انگلیسی مقاله ISI
Isolation, purification and characterization of a salt-active and organic-solvent-thermostable phenylalanine dehydrogenase from Bacillus nanhaiensis DSF-15A2
ترجمه فارسی عنوان
جداسازی، خالص سازی و تعیین خصوصیات دهیدروژناز فنیل آلانین مقاوم به حرارت ـ حلال ـ آلی و نمک فعال از nanhaiensis باسیلوس DSF-15A2
کلمات کلیدی
حلال تحمل آلی؛ نمک فعال؛ بیوکاتالیست؛ اسیدهای آمینه غیرطبیعی؛ شبیه سازی دینامیک مولکولی
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی کاتالیزور
چکیده انگلیسی


• A organic solvent-tolerant and salt-active BnPheDH with high activity was obtained.
• It showed better stability in methanol, ethanol, ethylene glycol, ethylene glycol monoethyl ether and acetone as compared to aqueous buffers.
• Affinity of the enzyme for the co-factor and substrate showed significant improvement in organic solvents.
• Catalytic efficiency i.e. kcat/Km showed significant improvement in the organic solvents.
• The BnPheDH’ stability could be significantly improved by 1 M NaCl.

BackgroundAs phenylalanine dehydrogenase (EC 1.4.1.20, PheDH) activity and stability was still diminished in organic solvent and biocatalytic reaction in non-aqueous media has presented as a great alternative to chemical synthesis and biosynthesis, suggesting that it is crucial to obtain a PheDH with high organic solvent-tolerant.ResultsIt is the first case when thermo-solvent tolerant PheDH producing bacterium was isolated from a marine sample. The BnPheDH’ stability could be significantly improved by 1 M NaCl, which was analyzed by molecular dynamics simulation, and it showed better stability in methanol, ethanol, ethylene glycol, ethylene glycol monoethyl ether and acetone. The kcat/Km in 30% (v/v) methanol and ethylene glycol monomethylether was 1.15- and 1.35-fold than that of in the aqueous phase, indicating that the catalytic efficiency was significantly improved by the methanol and ethylene glycol monomethylether.ConclusionsThe results indicated that the affinity of enzyme, substrate and co-factor and the catalytic efficiency can be significantly improved by organic solvents, suggesting that the BnPheDH had great application value in organic phase or aqueous-organic phase catalytic reaction, and it would be used as an robust biocatalyst to synthesize synthons in the food and pharmaceutical industries.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Molecular Catalysis B: Enzymatic - Volume 133, November 2016, Pages 12–19
نویسندگان
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