|کد مقاله||کد نشریه||سال انتشار||مقاله انگلیسی||ترجمه فارسی||نسخه تمام متن|
|69422||48763||2016||4 صفحه PDF||سفارش دهید||دانلود رایگان|
• A novel sulfate donor for the enzymatic sulfation of phenolic compounds is reported.
• This donor replaces the commonly used rather toxic P-nitrophenyl sulfate.
• Higher yields are obtained and purification of the sulfated compounds is simple.
An efficient and versatile donor for the sulfation by a bacterial arylsulfotransferase of various phenolic acceptor molecules is reported. Most studies in the past used toxic p-nitrophenyl sulfate as a sulfate donor for sulfation by this enzyme. However both the donor and p-nitrophenol are difficult to remove from the sulfated products. This new donor N-hydroxysuccinimide sulfate is easy to synthesize and has the advantage that at pH values above 7 it hydrolyzes to N-hydroxysuccinimide which is a safe compound and can easily be removed. As examples we demonstrated the formation of sulfated resveratrol and synthesized efficiently 3-sulfo-17-β-estradiol and bisphenol A bisulfate. It is likely that many other phenolic compounds are sulfated using this donor.
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Journal: Journal of Molecular Catalysis B: Enzymatic - Volume 129, July 2016, Pages 43–46