کد مقاله کد نشریه سال انتشار مقاله انگلیسی ترجمه فارسی نسخه تمام متن
69349 48752 2016 4 صفحه PDF سفارش دهید دانلود کنید
عنوان انگلیسی مقاله ISI
Enzymatic deglycosylation of flavonoids in deep eutectic solvents-aqueous mixtures: paving the way for sustainable flavonoid chemistry
ترجمه فارسی عنوان
deglycosylation آنزیمی از فلاونوئیدها در مخلوط یوتکتیک عمیق حلال آبی: هموار کردن راه برای شیمی فلاونوئید پایدار
کلمات کلیدی
فلاونون گلیکوزید؛ گلیسرول؛ اتیلن گلیکول؛ DES؛ Acremonium sp. DSM24697
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی کاتالیزور
چکیده انگلیسی


• Enzymatic flavonoid deglycosylation in DES and DES-components.
• Single reaction using a diglycosidase from Acremonium sp. DSM24697.
• A system for higher deglycosylation efficiency of hesperidin.
• Production of hesperetin.

The low solubility of glycosylated flavonoids represents a hurdle to conduct efficient enzymatic deglycosylations in aqueous media. To overcome this drawback, environmentally-unfriendly dimethylsulfoxide (DMSO) is typically used as co-solvent. Using a specific diglycosidase from Acremonium sp. DSM24697 for the deglycosylation of the rutinosylated flavonoid (hesperidin) as model reaction, this communication explores the use of (non-hazardous and biodegradable) deep eutectic solvents (DESs) as co-solvents in flavonoid biocatalysis. The enzymatic deglycosylation was observed when DES composed of choline chloride and glycerol or ethylene-glycol was used at proportions of up to 40% (DES-Buffer, v/v), displaying a promising framework to combine enhanced flavonoid solubilities and high enzymatic activities. The deglycosylation activity significantly increased when the single DES components – glycerol and ethylene-glycol – were added (e.g. 140% of enzyme activity at glycerol at 40% v/v), whereas deleterious effects were observed when choline chloride was solely added, presumably due to its chaotropic effect. Future research opportunities may be envisaged in the genetic design to evolve more robust biocatalysts, and in tailoring DES to deliver more enzyme-compatible solvents.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Molecular Catalysis B: Enzymatic - Volume 130, August 2016, Pages 70–73
نویسندگان
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