کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6964993 | 1452601 | 2018 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Biocatalytic production of adipic acid from glucose using engineered Saccharomyces cerevisiae
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Biocatalytic production of adipic acid from glucose using engineered Saccharomyces cerevisiae Biocatalytic production of adipic acid from glucose using engineered Saccharomyces cerevisiae](/preview/png/6964993.png)
چکیده انگلیسی
Adipic acid is an important industrial chemical used in the synthesis of nylon-6,6. The commercial synthesis of adipic acid uses petroleum-derived benzene and releases significant quantities of greenhouse gases. Biocatalytic production of adipic acid from renewable feedstocks could potentially reduce the environmental damage and eliminate the need for fossil fuel precursors. Recently, we have demonstrated the first enzymatic hydrogenation of muconic acid to adipic acid using microbial enoate reductases (ERs) - complex iron-sulfur and flavin containing enzymes. In this work, we successfully expressed the Bacillus coagulans ER in a Saccharomyces cerevisiae strain producing muconic acid and developed a three-stage fermentation process enabling the synthesis of adipic acid from glucose. The ability to express active ERs and significant acid tolerance of S. cerevisiae highlight the applicability of the developed yeast strain for the biocatalytic production of adipic acid from renewable feedstocks.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Metabolic Engineering Communications - Volume 6, June 2018, Pages 28-32
Journal: Metabolic Engineering Communications - Volume 6, June 2018, Pages 28-32
نویسندگان
Kaushik Raj, Siavash Partow, Kevin Correia, Anna N. Khusnutdinova, Alexander F. Yakunin, Radhakrishnan Mahadevan,