Immobilization-mediated reduction in melting temperatures of DNA-DNA and DNA-RNA hybrids: Immobilized DNA probe hybridization studied by SPR

- We report hybridization of DNA and RNA to a surface-bound DNA probe derived from TMV using SPR.
- For both RNA and DNA, the melting temperatures were ∼20 °C lower for solid state than in solution.
- Short oligomeric RNA targets may require measurement temperatures below room temperature.
- The melting temperature of the DNA:RNA hybrid was 17 °C lower than DNA:DNA duplex in the solid state.
- Hybrid melting temperatures are critical variables for biosensor oligonucleotide analysis.