Smart surface imprinting polymer nanospheres for selective recognition and separation of glycoprotein

- Surface imprinting nanosphere was prepared using covalent template immobilization.
- The imprinted nanospheres could rebind the template glycoprotein.
- The rebinding affinity of the imprinted nanospheres was thermo- and pH-dependent.
- High adsorption, fast rebinding kinetics, excellent selectivity and reusability.
- The applicability was favorable in a real sample analysis.