Rational design of didodecyldimethylammonium bromide-based nanoassemblies for gene delivery

- Three nanoassemblies based on a cheap cationic lipid, didodecyldimethylammonium bromide (DDAB) as gene vectors.
- The key components including oleic acid (OA), cholesterol (Chol), cholesteryl succinyl poly(ethylene glycol) 1500 (CHS-PEG), poly(d,l-lactide-co-glycolide)-methoxy-poly(ethylene glycol) (PLGA-PEG).
- The optimal component ratios in the nanoassemblies according to Langmuir monolayer behavior.
- Firstly compressing DNA or siRNA with the cationic lipid (i.e., DDAB) and secondly coated with the helper lipids (OA and CHS-PEG) of CPNA or the helper polymer (PLGA-PEG) of PPNA.
- CPNA and PPNA had the advantages of no cytotoxicity, high transfection efficiency, low cost and no sensitivity to serum.