کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
7066260 1459876 2018 13 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Intracellular carbonic anhydrase from Citrobacter freundii and its role in bio-sequestration
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی تکنولوژی و شیمی فرآیندی
پیش نمایش صفحه اول مقاله
Intracellular carbonic anhydrase from Citrobacter freundii and its role in bio-sequestration
چکیده انگلیسی
The aim of this work was to study the CO2 bio-sequestration application of indigenous Citrobacter species and its carbonic anhydrase (CA). Intracellular CA was purified from Citrobacter freundii (CF; accession no: MH283871) isolated from limestone rock site in Kumaun region of Indian Himalaya studied for the sequestration of carbon dioxide and the formation of calcite. CF showed maximum CA enzyme activity at 11.3 EU/ml at pH 7.0 and 37 °C. Hydration of CO2 into carbonate was characterized by calcite phase of calcium carbonate using absorption spectroscopy and imaging technique. Purified CA showed a significantly high CO2 sequestration capacity of 230 mg CaCO3/mg of purified as compared to crude enzyme (50 mg CaCO3/ml of enzyme).
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Bioresource Technology - Volume 267, November 2018, Pages 789-792
نویسندگان
, , , ,