کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
7068425 1459890 2018 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Induction of calcite precipitation through heightened production of extracellular carbonic anhydrase by CO2 sequestering bacteria
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی تکنولوژی و شیمی فرآیندی
پیش نمایش صفحه اول مقاله
Induction of calcite precipitation through heightened production of extracellular carbonic anhydrase by CO2 sequestering bacteria
چکیده انگلیسی
The thermo-alkalotolerant bacterium exhibiting heightened extracellular carbonic anhydrase (CA) activity, survived at 100 mM sodium bicarbonateand 5% gaseous CO2 was identified as Bacillus sp. by 16S rRNA sequencing. Extracellular carbonic anhydrase was purified by ammonium sulfate precipitation, gel filtration chromatography and affinity chromatography with a yield of 46.61% and specific activity of 481.66 U/mg. The size of purified carbonic anhydrase was approximately 28 kDa in SDS-PAGE gel filtration and further their role in calcium carbonate production was correlated. The purified enzyme was stable with half-life of 25.36 min at 90 °C and pH 8. KM and Vmax values of the enzyme were 1.77 mg/mL and 385.69 U/mg respectively. The production of calcite was confirmed by Scanning Electron Microscopy (SEM) analysis, FTIR, and Energy-Dispersive X-ray (EDX) analysis. Carbonic anhydrase and calcite deposition coupled with CO2 fixingbacteria is a significant approach for CO2 sequestration.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Bioresource Technology - Volume 253, April 2018, Pages 368-371
نویسندگان
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