کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7070323 | 1459925 | 2016 | 33 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Anaerobic and sequential aerobic production of high-titer ethanol and single cell protein from NaOH-pretreated corn stover by a genome shuffling-modified Saccharomyces cerevisiae strain
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
تکنولوژی و شیمی فرآیندی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
In this study, a Saccharomyces cerevisiae recombinant strain 14 was constructed through genome shuffling method by transferring the whole genomic DNA of Candida intermedia strain 23 into a thermo-tolerant S. cerevisiae strain. The recombinant strain 14 combined the good natures of both parent strains that efficiently produced ethanol from glucose and single cell protein from xylose with 54.6% crude protein and all essential amino acids except cysteine at 35 °C. Importantly, the recombinant strain 14 produced 64.07 g/L ethanol from 25% (w/v) NaOH-pretreated and washed corn stover with the ethanol yield of 0.26 g/g total stover by fed-batch simultaneous saccharification and fermentation and produced 66.50 g/L dry cell mass subsequently from the residual hydrolysate and ethanol. Therefore, this study represents a feasible method to comprehensively utilize hexose and pentose in lignocellulosic materials.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Bioresource Technology - Volume 218, October 2016, Pages 623-630
Journal: Bioresource Technology - Volume 218, October 2016, Pages 623-630
نویسندگان
Xueliang Ren, Juncong Wang, Hui Yu, Chunlan Peng, Jinlong Hu, Zhiyong Ruan, Shumiao Zhao, Yunxiang Liang, Nan Peng,