کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
7078964 1459987 2014 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Construction of reductive pathway in Saccharomyces cerevisiae for effective succinic acid fermentation at low pH value
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی تکنولوژی و شیمی فرآیندی
پیش نمایش صفحه اول مقاله
Construction of reductive pathway in Saccharomyces cerevisiae for effective succinic acid fermentation at low pH value
چکیده انگلیسی
Succinic acid is an important precursor for the synthesis of high-value-added products. Saccharomyces cerevisiae is a suitable platform for succinic acid production because of its high tolerance towards acidity. In this study, a modified pathway for succinate production was established and investigated in S. cerevisiae. The engineered strain could produce up to 6.17 ± 0.34 g/L of succinate through the constructed pathway. The succinate titer was further improved to 8.09 ± 0.28 g/L by the deletion of GPD1 and even higher to 9.98 ± 0.23 g/L with a yield of 0.32 mol/mol glucose through regulation of biotin and urea levels. Under optimal supplemental CO2 conditions in a bioreactor, the engineered strain produced 12.97 ± 0.42 g/L succinate with a yield of 0.21 mol/mol glucose at pH 3.8. These results demonstrated that the proposed engineering strategy was efficient for succinic acid production at low pH value.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Bioresource Technology - Volume 156, March 2014, Pages 232-239
نویسندگان
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