کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7078964 | 1459987 | 2014 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Construction of reductive pathway in Saccharomyces cerevisiae for effective succinic acid fermentation at low pH value
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
تکنولوژی و شیمی فرآیندی
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چکیده انگلیسی
Succinic acid is an important precursor for the synthesis of high-value-added products. Saccharomyces cerevisiae is a suitable platform for succinic acid production because of its high tolerance towards acidity. In this study, a modified pathway for succinate production was established and investigated in S. cerevisiae. The engineered strain could produce up to 6.17 ± 0.34 g/L of succinate through the constructed pathway. The succinate titer was further improved to 8.09 ± 0.28 g/L by the deletion of GPD1 and even higher to 9.98 ± 0.23 g/L with a yield of 0.32 mol/mol glucose through regulation of biotin and urea levels. Under optimal supplemental CO2 conditions in a bioreactor, the engineered strain produced 12.97 ± 0.42 g/L succinate with a yield of 0.21 mol/mol glucose at pH 3.8. These results demonstrated that the proposed engineering strategy was efficient for succinic acid production at low pH value.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Bioresource Technology - Volume 156, March 2014, Pages 232-239
Journal: Bioresource Technology - Volume 156, March 2014, Pages 232-239
نویسندگان
Daojiang Yan, Caixia Wang, Jiemin Zhou, Yilan Liu, Maohua Yang, Jianmin Xing,