کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7079457 | 1459992 | 2014 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Mycelium differentiation and development of Streptomyces coelicolor in lab-scale bioreactors: Programmed cell death, differentiation, and lysis are closely linked to undecylprodigiosin and actinorhodin production
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کلمات کلیدی
OTRStreptomycesPMTPCDAntibiotics - آنتی بیوتیک هاBioreactor - بیورآکتور یا رآکتور زیستیDifferentiation - تفکیکdissolved oxygen tension - تنش اکسیژن حل شدهphotomultiplier tube - لوله فوتومولتیپلیرOUR - ماProgrammed cell death - مرگ برنامهریزی شده یاختهOxygen uptake rate - میزان جذب اکسیژنDOT - نقطهMII - هزارPropidium iodide - پروتئین یدید
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
تکنولوژی و شیمی فرآیندی
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چکیده انگلیسی
Streptomycetes are mycelium-forming bacteria that produce two thirds of clinically relevant secondary metabolites. Secondary metabolite production is activated at specific developmental stages of Streptomyces life cycle. Despite this, Streptomyces differentiation in industrial bioreactors tends to be underestimated and the most important parameters managed are only indirectly related to differentiation: modifications to the culture media, optimization of productive strains by random or directed mutagenesis, analysis of biophysical parameters, etc. In this work the relationship between differentiation and antibiotic production in lab-scale bioreactors was defined. Streptomyces coelicolor was used as a model strain. Morphological differentiation was comparable to that occurring during pre-sporulation stages in solid cultures: an initial compartmentalized mycelium suffers a programmed cell death, and remaining viable segments then differentiate to a second multinucleated antibiotic-producing mycelium. Differentiation was demonstrated to be one of the keys to interpreting biophysical fermentation parameters and to rationalizing the optimization of secondary metabolite production in bioreactors.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Bioresource Technology - Volume 151, January 2014, Pages 191-198
Journal: Bioresource Technology - Volume 151, January 2014, Pages 191-198
نویسندگان
Beatriz Rioseras, MarÃa Teresa López-GarcÃa, Paula Yagüe, Jesús Sánchez, Ángel Manteca,