Carrier free co-immobilization of alpha amylase, glucoamylase and pullulanase as combined cross-linked enzyme aggregates (combi-CLEAs): A tri-enzyme biocatalyst with one pot starch hydrolytic activity

- Development of combi-CLEAs of alpha amylase, glucoamylase and pullulanase.
- Combi-CLEAs improved optimal conditions for one pot starch hydrolysis.
- Co-immobilization as combi-CLEAs enhanced thermal stability of enzymes.
- One pot starch hydrolysis in batch mode using combi-CLEAs was studied.
- Reusability of enzymes during one pot starch hydrolysis was determined.