کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7081953 | 1460001 | 2013 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Comparison of process configurations for ethanol production from acid- and alkali-pretreated corncob by Saccharomyces cerevisiae strains with and without β-glucosidase expression
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
تکنولوژی و شیمی فرآیندی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
β-Glucosidase was shown to have synergistic effects with commercial cellulase in the hydrolysis of acid- and alkali-pretreated corncob, especially at the dose of 5 U/g biomass and 5 or 10 FPU/g biomass. An integrating yeast strain 45# expressing β-glucosidase was constructed that utilized cellobiose quickly and efficiently. Process configurations were compared under conditions of 10% solid content, 10 FPU cellulase/g biomass, 5 U β-glucosidase/g biomass (only used for parental strain W303-1A), 1 g/kg yeast loading and 3.3 g/kg urea supplementation. While separate hydrolysis and fermentation was optimal for W303-1A and the ethanol titer and yield reached 3.22 g/100 g and 75.6% (expressed as a percentage of the theoretical yield), respectively, simultaneous saccharification and fermentation was optimal for strain 45# and the ethanol titer and yield reached 3.31 g/100 g and 77.7%, respectively. These results are valuable in optimization of the process configuration and improving the yeast strain selected for cellulosic ethanol production.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Bioresource Technology - Volume 142, August 2013, Pages 154-161
Journal: Bioresource Technology - Volume 142, August 2013, Pages 154-161
نویسندگان
Guoqiang Wang, Cheng Liu, Jiefang Hong, Yuanyuan Ma, Kun Zhang, Xinyu Huang, Shaolan Zou, Minhua Zhang,