Use of artificial neural network (ANN) for the development of bioprocess using Pinus roxburghii fallen foliages for the release of polyphenols and reducing sugars

- Pretreatment of pine needle was optimized by ANN for yield of polyphenol and sugar.
- 6 ml (0.5% NaOH)/g of substrate, 10 min soaking, 1 min microwave were optimal.
- Cocktail of enzymes yielded 669 mg/g of sugar and 265 mg/g of polyphenols.
- This work is of own kind where ANN was used for optimization of extraction of phythochemicals and sugars.
- Pine wastes can be used in the biorefinery system to get various value added products.