کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7082398 | 1460001 | 2013 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Heterologous expression and characterization of a novel thermo-halotolerant endoglucanase Cel5H from Dictyoglomus thermophilum
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
تکنولوژی و شیمی فرآیندی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
A novel β-1,4-endoglucanase gene was cloned from Dictyoglomus thermophilum, designated as Cel5H for being a member of glycoside hydrolase family 5. The purified recombinant endoglucanase showed high hydrolytic activities on carboxylmethyl cellulose with a broad optimal temperature of 50-85 °C and an optimal pH of 5.0. Furthermore, this enzyme was highly thermostable with a half-life of 336 h at 70 °C and retained more than 80% of the initial activity after 135 days incubation at 50 °C. To enhance the performance of the thermophilic endoglucanase, chimeric enzymes containing Cel5H and syncretic cellulose binding module (CBM) were constructed. The results showed that all the CBMs were effective. In addition, Cel5H was highly tolerant against high salt concentration and distinguished from salt-tolerant bacteria since it was independent of high salt concentration. Three-dimensional structure of Cel5H was developed by homology modeling methods and surface electrostatic analysis was performed.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Bioresource Technology - Volume 142, August 2013, Pages 338-344
Journal: Bioresource Technology - Volume 142, August 2013, Pages 338-344
نویسندگان
Runrun Shi, Zhimin Li, Qin Ye, Jianhe Xu, Yan Liu,