A biorefinery from Nannochloropsis sp. microalga - Extraction of oils and pigments. Production of biohydrogen from the leftover biomass

► Nannochloropsis sp. was studied as a biorefinery context. ► Production of fatty acids for biodiesel, high added-value compounds and biohydrogen. ► Extracts fractionation by SFE (oils for biodiesel and pigments for food). ► Fermentation of biomass leftover by E. aerogenes yielded maximum 60.6 mL H2/gdry biomass alga. ► Best SFE conditions extracted 45 glipids/100 gdry biomass (40 °C, 300 bar, CO2 + ethanol).