Production of biodiesel from plant oil hydrolysates using an Aspergillus oryzae whole-cell biocatalyst highly expressing Candida antarctica lipase B

► Enzymatic production of biodiesel from plant oil hydrolysates. ► The catalyst was an immobilized recombinant fungus expressing CALB (r-CALB). ► Esterification using r-CALB attained a methyl ester content over 90% after 6 h. ► Stepwise additions of methanol and a little water were unnecessary. ► A methyl ester content over 90% was maintained during 20 batch cycles.