Fluorescence detection to determine proteins and humic-like substances fingerprints of exopolymeric substances (EPS) from biological sludges performed by size exclusion chromatography (SEC)

► EPS were studied by size exclusion chromatography with UV and fluorescence detection. ► Fluorescence detection allows to obtain specific proteins and humic-like substances fingerprints. ► Changes in peak intensity and number are highlighted between UV and fluorescence detection. ► EPS display a protein-like fraction with a wide range of aMW (from >600 kDa to <10 kDa). ► EPS show a humic-like fraction with low aMW (from 6 kDa to <1.2 kDa).