Inhibitory effect of lignin during cellulose bioconversion: The effect of lignin chemistry on non-productive enzyme adsorption

► Lignin was isolated from pretreated and native spruce and wheat straw. ► Homogeneous, ultrathin lignin films were successfully generated by spin-coating. ► Monocomponent cellulase adsorption on the lignin films was studied with QCM. ► Highest cellulase adsorption was detected on pretreated lignin films. ► Lower lignin-binding was detected when using enzyme without CBM and linker domain.