Stoichiometry and kinetics of the PHB-producing Type II methanotrophs Methylosinus trichosporium OB3b and Methylocystis parvus OBBP

► PHB production by Type II methanotrophs is of commercial and environmental interest. ► We model stoichiometry and kinetics of Type II methanotrophs. ► We show effects of oxygen and nitrogen sources on Type II methanotrophic growth. ► Methane requirement to produce PHB is a key parameter and organism-specific. ► Analyses can inform bioreactor design, scale-up models, and life cycle assessments.