Aerobic metabolism of mixed carbon sources in sequencing batch reactor under pulse and continuous feeding

► Aerobic storage of PHB and glycogen from acetate/starch mixture was studied. ►13C NMR analysis was used to evaluate metabolic pathways of aerobic storage. ► Metabolism of glycogen from starch was not affected by the presence of acetate. ► Starch strongly affected acetate metabolism via anaplerotic and catabolic routes. ► PHB storage from acetate strongly decreased in the presence of starch.