Construction of an integrated enzyme system consisting azoreductase and glucose 1-dehydrogenase for dye removal

► NADH regeneration system was created by azoreductase and glucose 1-dehydrogenase. ► 1 U azoreductase:10 U glucose 1-dehydrogenase was the most suitable enzyme ratio. ► Artificial neural network could satisfactory fit for integrated enzyme system. ► All variables have strong effects on dye removal of integrated enzyme system. ► Batch results indicated integrated enzyme system was potential for dye removal.