Enhancement of 2-keto-gulonic acid yield by serial subcultivation of co-cultures of Bacillus cereus and Ketogulonigenium vulgare

► The yield of 2-KGA was enhanced by serial subcultivation.► Subcultivated co-culture increased the 2-KGA yield from 77% to 93%. ► Serial subcultivation is of industrial interest for vitamin C production.