Sugarcane bagasse ozonolysis pretreatment: Effect on enzymatic digestibility and inhibitory compound formation

► Ozonolysis in fixed bed is an efficient sugarcane bagasse pretreatment. ► Sample moisture and ozone concentration have a major impact on ozonolysis. ► Ozonolysis causes low carbohydrate degradation and few inhibitory compounds. ► Water washing is an effective detoxification alternative for bagasse ozonolysis. ► Electronic microscopy cleared morphological changes in bagasse.