Evaluation of FT-IR and Nile Red methods for microalgal lipid characterization and biomass composition determination

- FT-IR and Nile Red were compared with gravimetry for microalgal lipid determination.
- Carbohydrate and protein measured by FT-IR were compared with DNS and Bradford.
- Nile Red staining could locate intracellular and extracellular lipid droplets.
- Guideline of methods selection for microalgal lipid characterization is proposed.