Two neutral thermostable cellulases from Phialophora sp. G5 act synergistically in the hydrolysis of filter paper

► Two genes of family 6 and 45 cellulases were cloned from Phialophora sp. G5. ► The neutral enzymes produced in Pichia pastoris had high activities over pH 4.0-10.0. ► Both enzymes had excellent pH stability and thermostability and SDS resistance. ► The two enzymes had significant synergism in hydrolysis of filter paper. ► The enzyme mixture shows application potentials in detergent and textile industries.