Biochemical methane potential of microalgae: Influence of substrate to inoculum ratio, biomass concentration and pretreatment

► Anaerobic digestion of microalgae have highest productivities at S/I ratios of 0.5. ► Methane productivity is depended of microalgae specie. ► The microalgae concentration for anaerobic digestion must be greater than 10 gTS/kg. ► COD solubilization no imply an improvement on methane productivity. ► Thermal hydrolysis substantially improves methane productivity of microalgae.