Study of the interactive effect of temperature and pH on exopolysaccharide production by Enterobacter A47 using multivariate statistical analysis

▸ Enterobacter A47 was cultivated at different temperature and pH values. ▸ RSM was used to evaluate the effect on cell growth and EPS synthesis. ▸ Cell growth was favored at pH < 7.0. ▸ EPS production was maximal for 25 < T < 35 °C and 6.0 < pH < 8.0. ▸ Distinct sugar and acyl groups composition were obtained.