Reed canary grass as a feedstock for 2nd generation bioethanol production

► Hydrolysis of reed canary grass harvested in the spring and autumn was compared. ► Additional β-glucosidase was shown to improve hydrolysis of cellulose and xylan. ► Supplementary CBHII increased the hydrolysis level of RCG by 10%. ► 82% ethanol yield was obtained on steam pretreated RCG.