Optimisation of glycogen quantification in mixed microbial cultures

► The method for glycogen analysis is optimised using design of experiments. ► Different methods should be used for floccular and granular cultures. ► The biomass concentration should be as low as possible, i.e. 1 mg/mL. ► The acid concentration should be increased to 0.9 M HCl. ► A global method can be used when the granulation is unknown or unstable.