Development of a calibration protocol and identification of the most sensitive parameters for the particulate biofilm models used in biological wastewater treatment

► A calibration protocol of the attached growth and biofilm models is developed. ► The proposed protocol sets a complete strategy to model biofilm reactors. ► The proposed protocol sets a method to collect a useful biofilm data. ► The proposed protocol sets a method to plan sampling and monitoring requirements. ► Sensitivity analysis is a very helpful tool to guide experimental measurements.