کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7141988 | 1462034 | 2018 | 24 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Direct detection of organophosphate compounds in water by a fluorescence-based biosensing device
ترجمه فارسی عنوان
تشخیص مستقیم ترکیبات ارگانوفسفره در آب بوسیله دستگاه بیوسنسین بر پایه فلورسانس
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کلمات کلیدی
tri-n-butylphosphineEST2PECQDsTBPVMDp-NP - p-npp-nitrophenyl - p-نیترفنیلAChE - آهیOrganophosphate - ارگانوفسفاتOrganophosphates - ارگانوفسفاتThermophilic esterase - استراز ترموفیلAcetylcholinesterases - استیل کولین استرازLOD یا Limit of detection - حد تشخیصvisual molecular dynamics - دینامیک مولکولی بصریFlow cell - سلول جریانPhotoelectrochemical - فوتو الکتروشیمیاییlimits of detection - محدودیت های تشخیصquantum dots - نقاط کوانتومیParaoxon - پاراکسونFluorescent probe - پروب فلورسنتIAEDANS - یادانس
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
The main drawbacks in the use of acetylcholinesterase-based biosensors are their susceptibility to inhibition by too many chemicals, their limited time-stability, and the constant need for a supply of substrates for the measurements. In order to offset these deficiencies, we have addressed our studies towards the thermophilic esterase 2 from A. acidocaldarious, which shows a high specificity and affinity towards organophosphates and a high resistance under raw operative conditions. In particular, we have investigated the possibility of measuring the binding of organophosphates to the protein by using a fluorescent probe covalently linked near the active site. We have produced a mutant where the serine 35, a residue located at the entrance of the alcohol binding site, has been replaced by a cysteine residue. The addition of 1,5-IAEDANS as a fluorescent probe to the thiol group of the mutant-protein did not affect the capability of the enzyme to bind the paraoxon and its stability or instability over time. We have set up a continuous flow system based on a re-circulating solution of the probe-enzyme complex through a fluorimetric flow cell inside a spectrofluorimeter. The addition of paraoxon aliquots has been detected in real-time by measuring the fluorescence quenching of the probe-enzyme complex. The fluorescence signals, as well as the enzyme activity, were not affected by dilution and organic solvent addition. These results support the development of biosensing devices for the continuous monitoring of organophosphate compounds.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Sensors and Actuators B: Chemical - Volume 255, Part 3, February 2018, Pages 3257-3266
Journal: Sensors and Actuators B: Chemical - Volume 255, Part 3, February 2018, Pages 3257-3266
نویسندگان
Paola Carullo, Marco Chino, Giovanni Paolo Cetrangolo, Sara Terreri, Angela Lombardi, Giuseppe Manco, Amelia Cimmino, Ferdinando Febbraio,