کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
7228949 1470914 2018 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
A solid ionic Lactate biosensor using doped graphene-like membrane of Au-EVIMC-titania nanotubes-polyaniline
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
A solid ionic Lactate biosensor using doped graphene-like membrane of Au-EVIMC-titania nanotubes-polyaniline
چکیده انگلیسی
Herein, a doped graphene-like membrane was designed, which was copolymerized to be a solid ionic biosensor by using titania nanotubes (TiNTs), polyaniline (PANI), EvimCl (1-ethyl-3-vinylimidazolium chloride, EVIMC) and chloroauric acid (HAuCl4). The structure of graphene-like arrangement and the copolymerization mechanism of the film were discussed in detail. Because of the high catalytic property, Lactate could be determined on the membrane catalyzed by lactate dehydrogenase (LDH) containing isocitrate dehydrogenase (NAD+), which was immobilized onto the film by electrostatic attraction. The electrochemical response on the LDH/Au-EVIMC-TiNTs-PANI/ITO was increased by twice than the LDH/TiNTs/PANI/ITO, and exhibited two linear responses within the concentration range from 5.5 × 10−7 M to 5.55 × 10−6 M, and 5.55 × 10−6 M to 3.33 × 10−3 M, with a detection limit of 1.65 × 10−7 M (S / N = 3). The interference study for other common coexistence such as uric acid and hemoglobin revealed that there was no overlapping signal for the detection of Lactate on the biosensor. The developed method proved the most stability in the determination of real blood samples, and the recoveries ranged from 96.7% to 105.8% with a satisfactory result.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biosensors and Bioelectronics - Volume 118, 30 October 2018, Pages 97-101
نویسندگان
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