کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7229057 | 1470915 | 2018 | 27 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
In situ enzymatic generation of gold for ultrasensitive amperometric sandwich immunoassay of procalcitonin
ترجمه فارسی عنوان
در محل تولید آنزیمی طلا برای سنجش حساسیت آمپولومتری ساندویچ پروکلسیتونین
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
Procalcitonin (PCT) is an important indicator for bacterial inflammatory diseases, and its sensitive, accurate and rapid detection has important clinical value. On the basis of sandwich immunoassay, glucose oxidase-catalyzed gold deposition and in-situ microliter-droplet anodic stripping voltammetry (ASV) of the enzyme-generated gold directly on the immunoelectrode, the ultrasensitive electrochemical detection of PCT is achieved. A new method of the chemical dissolution of gold by an appropriately diluted aqua regia and the simultaneous cathodic preconcentration of gold on the immunoelectrode is suggested, which gives the better performance for the ASV analysis of gold than the reported one. Under optimized conditions, the ASV peak current is linear with the common logarithm of PCT concentration from 0.05â¯fgâ¯mLâ1 to 500â¯ngâ¯mLâ1, with a limit of detection (LOD, S/Nâ¯=â¯3) as low as 0.04â¯fgâ¯mLâ1. Our method has also been used for detection of PCT in serum samples with satisfactory results.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biosensors and Bioelectronics - Volume 117, 15 October 2018, Pages 422-428
Journal: Biosensors and Bioelectronics - Volume 117, 15 October 2018, Pages 422-428
نویسندگان
Yuyun Sui, Aigui Xu, Xiaorui Jin, Jiao Zheng, Xin He, Yan Cheng, Qingji Xie, Rushi Liu,