کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7229566 | 1470924 | 2018 | 24 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Dual-reaction triggered sensitivity amplification for ultrasensitive peptide-cleavage based electrochemical detection of matrix metalloproteinase-7
ترجمه فارسی عنوان
واکنش دوگانه موجب تقویت حساسیت برای شناسایی الکتروشیمیایی مبتنی بر پپتید فوقانی حساس پالایش ماتریکس متالوپروتئیناز 7
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کلمات کلیدی
ماتریکس متالوپروتئیناز 7، بیوسنسور الکتروشیمیایی، 4-کلرو-1-نافول، اختلال پپتید، هیدروژل آلژینات سدیم،
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
In this work, a new strategy of dual-reaction triggered sensitivity amplification for ultrasensitive electrochemical detection of matrix metalloproteinase-7 (MMP-7) was developed. The sensitivity of amperometric biosensor relies on the current signal differences (ÎI) caused by per unit concentration target. Benefited from dual-reaction catalytic activities of Pd nanoparticles, dual catalytic reactions were implemented in the biosensor to amplify the ÎI: (1) Fenton-like reaction was triggered by the probes to degrade redox species methylene blue; (2) catalytic precipitation reaction was followed subsequently to generate insoluble precipitation by 4-chloro-1-naphthol oxidation. Dual-enhancement of ÎI triggered by Pd nanoparticle-based catalytic probes significantly improved the detection performance of the biosensor. The peptide-cleavage based biosensor integrated Pd nanoparticle-based catalytic probes with reduced graphene oxide-Au/methylene blue-sodium alginate hydrogel (Au-rGO/MB-SA) nanocomposites substrate for ultrasensitive detection of MMP-7. Under optimal conditions, the proposed biosensor exhibited a wide linear range from 10â¯fgâ¯mLâ1 to 10â¯ngâ¯mLâ1 with an ultralow detection limit of 3.1â¯fgâ¯mLâ1. This strategy successfully combines the multiple catalytic reactions triggered by nanomaterials with peptide-cleavage pattern in electrochemical biosensor, providing a promising method for detection of other proteases.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biosensors and Bioelectronics - Volume 108, 15 June 2018, Pages 46-52
Journal: Biosensors and Bioelectronics - Volume 108, 15 June 2018, Pages 46-52
نویسندگان
Yun Zheng, Zhanfang Ma,