کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7229624 | 1470926 | 2018 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Selected landscape phage probe as selective recognition interface for sensitive total prostate-specific antigen immunosensor
ترجمه فارسی عنوان
فایگ لنزهای چشم انداز انتخاب شده به عنوان رابط تشخیص انتخابی برای حساسیت کل ایمنی حساس به آنتی ژن خاص پروستات
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کلمات کلیدی
فاژ پروب، رابط شناختی کل آنتی ژن اختصاصی پروستات، ایمونوسانسور الکتروشیمیایی، فاژ الیزا،
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
The level of total prostate-specific antigen (t-PSA) is generally known as the key index of prostate cancer. Here, phage probes against t-PSA were selected from f8/8 landscape phage library. After three rounds of biopanning, four t-PSA-binding phage clones were isolated and identified by the DNA sequencing. Based on the phage capture assay, the phage clone displaying the fusion peptide ATRSANGM showed highest affinity and specificity against t-PSA. Subsequently, the t-PSA-specific phage was used as t-PSA capture probe in a sandwich enzyme-linked immunosorbent assay (ELISA) and differential pulse voltammetry (DPV) assay systems. Both assay methods showed high specificity and acceptable reliability for real serum samples analysis. By comparison, DPV method showed wider linear range (0.01-100â¯ngâ¯mLâ1) and lower limit of detection (3â¯pgâ¯mLâ1) than those (3.3-330â¯ngâ¯mLâ1 and 1.6â¯ngâ¯mLâ1) of ELISA. Moreover, DPV system showed smaller distinction to the authoritative method in real samples assay. Excitingly, the phage probe based DPV immunosensor showed high sensitivity for the detection of t-PSA and LOD achieved the pgâ¯mLâ1 level, which was far lower than those values (usually above 0.1â¯ngâ¯mLâ1) for reported immunosensors based on antibodies. Due to the biocompatibility, multivalency, stability, and high structural homogeneity, the t-PSA-specific landscape phage demonstrates as a novel specific interface in biosensors.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biosensors and Bioelectronics - Volume 106, 30 May 2018, Pages 1-6
Journal: Biosensors and Bioelectronics - Volume 106, 30 May 2018, Pages 1-6
نویسندگان
Lei Han, Hongqi Xia, Long Yin, Valery A. Petrenko, Aihua Liu,